CRYSTAL STRUCTURE ANALYSIS OF THE E. COLI MANGANESE SUPEROXIDE DISMUTASE Y34F MUTANT

Outer sphere mutations perturb metal reactivity in manganese superoxide dismutase.

Removing a hydrogen bond in the dimer interface of Escherichia coli manganese superoxide dismutase alters structure and reactivity.

Crystal Structure of Escherichia coli Manganese Superoxide Dismutase at 2.1 A Resolution

SYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTFVNNANAALESLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHAN
HSLFWKGLKKGTTLQGDLKAAIERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGASG
FPIMGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK

20% w/v PEG 6000, 0.1 M bicine, 25 mM sodium azide, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K

iron-substituted native crystal structure

E. coli manganese superoxide dismutase active-site Q146H mutant crystal structure

E. coli manganese superoxide dismutase active-site Q146L mutant crystal structure

proton shuttle, Y34F, mutant, manganese superoxide dismutase, OXIDOREDUCTASE