CRYSTAL STRUCTURE OF HUMAN BRANCHED CHAIN AMINO ACID AMINOTRANSFERASE (MITOCHONDRIAL) COMPLEXED WITH PYRIDOXAL-5'-PHOSPHATE AT 2.5 ANGSTROMS (MONOCLINIC FORM).

The structure of human mitochondrial branched-chain aminotransferase.

Cloning of the Rat and Human Mitochondrial Branched Chain Aminotransferases (BCATm)

BRANCHED CHAIN AMINO ACID AMINOTRANSFERASE (MITOCHONDRIAL)

ASSSFKAADLQLEMTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKA
FKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPRR
ALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGT
MNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPV
HRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

PEG1500, DTT, HEPES;
NA OR K PHOSPHATE, DTT, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

1EKF CONTAINS THE SAME PROTEIN IN ORTHORHOMBIC FORM COMPLEXED WITH PYRIDOXAL PHOSPHATE

1EKV CONTAINS THE SAME PROTEIN INACTIVATED BY TRIS BOUND TO THE PYRIDOXAL PHOSPHATE ON ONE END AND ACTIVE SITE LYS202 NZ ON THE OTHER.

E. COLI BRANCHED CHAIN AMINO ACID AMINOTRANSFERASE

THE BIOLOGICAL ASSEMBLY IS A DIMER CONSTRUCTED FROM CHAINS A AND B.