CRYSTAL STRUCTURE OF RAT MINOR HISTOCOMPATIBILITY ANTIGEN COMPLEX RT1-AA/MTF-E.

Two different, highly exposed, bulged structures for an unusually long peptide bound to rat MHC class I RT1-Aa.

Identification of the rat maternally transmitted minor histocompatibility antigen

Efficient generation of major histocompatibility complex class I-peptide complexes using synthetic peptide libraries

Peptide length preferences for rat and mouse MHC class I molecules using random peptide libraries

The rat cim effect: TAP allele-dependent changes in a class I MHC anchor motif and evidence against C-terminal trimming of peptides in the ER

An analysis of the antigen binding site of RT1-Aa suggests an allele-specific motif

CLASS I MAJOR HISTOCOMPATIBILITY ANTIGEN RT1-AA

GSHSLRYFYTAVSRPGLGEPRFIAVGYVDDTEFVRFDSDAENPRMEPRARWMEREGPEYWEQQTRIAKEWEQIYRVDLRT
LRGYYNQSEGGSHTIQEMYGCDVGSDGSLLRGYRQDAYDGRDYIALNEDLKTWTAADFAAQITRNKWERARYAERLRAYL
EGTCVEWLSRYLELGKETLLRSDPPEAHVTLHPRPEGDVTLRCWALGFYPADITLTWQLNGEDLTQDMELVETRPAGDGT
FQKWASVVVPLGKEQNYTCRVEHEGLPKPLSQRWE

IQKTPQIQVYSRHPPENGKPNFLNCYVSQFHPPQIEIELLKNGKKIPNIEMSDLSFSKDWSFYILAHTEFTPTETDVYAC
RVKHVTLKEPKTVTWDRDM

0.1M morpholine ethansulfonic acid (MES),
0.2M ammonium sulfate,
15-20% MPEG 5000,
0.025M beta-octyl-glucoside,
0.5% glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K

Engh & Huber as implemented in X-PLOR and CNS

There are two heterotrimeric biological assemblies in the asymmetric unit constructed from chains A, B, and C, or chains D, E, and F.

major histocompatibility complex, rat minor histocompatibility complex, MHC, immunology, peptide antigen presentation, cellular immunity, cell surface receptor, T cell receptor ligand, IMMUNE SYSTEM