NMR SOLUTION STRUCTURE OF ALPHA-CONOTOXIN IM1 POINT MUTATION VARIANT R11E
Structure-Activity Relationships in a Peptidic Alpha7 Nicotinic Acetylcholine Receptor Antagonist
NMR Solution Structure of Alpha-Conotoxin Imi and Comparison to Other Conotoxins Specific for Neuronal Nicotinic Acetylcholine Receptors
Alpha-Conotoxin Imi Exhibits Subtype-Specific Nicotinic Acetylcholine Receptor Blockade: Preferential Inhibition of Homomeric Alpha7 and Alpha9 Receptors
A Nicotinic Acetylcholine Receptor Ligand of Unique Specificity, Alpha-Conotoxin Imi
NMR SOLUTION STRUCTURE OF ALPHA-CONOTOXIN IM1 POINT MUTATION VARIANT R7L (20 STRUCTURES)
NMR SOLUTION STRUCTURE OF ALPHA-CONOTOXIN IM1 POINT MUTATION VARIANT D5N (20 STRUCTURES)
NMR SOLUTION STRUCTURE OF ALPHA-CONOTOXIN IM1, (20 STRUCTURES)
SOLUTION STRUCTURE OF ALPHA-CONOTOXIN IM1
SYNTHESIZED USING STANDARD FMOC CHEMISTRY. IM1 SEQUENCE FOUND NATURALLY IN CONUS IMPERIALIS VENOM
DATA CONSIST OF 61 UPPER LIMITS ON DISTANCES OBTAINED FROM NOE MEASUREMENTS AND 26 ANGLE CONSTRAINTS OBTAINED FROM NOE MEASUREMENTS AND COUPLING CONSTANT MEASUREMENTS. THESE INPUT DATA ARE ALSO AVAILABLE FROM THE PROTEIN DATA BANK. ONE STEREOSPECIFIC RESONANCE ASSIGNMENT HAS BEEN MADE. TORSION ANGLE DYNAMICS CALCULATIONS WERE PERFORMED WITH THE PROGRAM DYANA (P. GUNTERT, C. MUMENTHALER, K. WUTHRICH, J. MOL. BIOL. (1997) VOL. 273, 283-298). FOR THE RESTRAINED ENERGY MINIMIZATION THE PROGRAM OPAL (P. LUGINBUHL, P. GUNTERT, M. BILLETER, K. WUTHRICH, J. BIOMOL. NMR (1996) VOL. 8, 136-146) WAS USED. DEPOSITED COORDINATES ARE THOSE OF CONFORMERS 1-20 IN THE PAPER CITED ON *JRNL* RECORDS ABOVE. NO VIOLATIONS OF DISTANCE CONSTRAINTS FROM NOES EXCEED 0.10 ANGSTROMS, AND NO VIOLATIONS OF ANGLE CONSTRAINTS EXCEED 2.0 DEGREES. REPRESENTATIVE CONFORMER HAS THE SMALLEST RMSD TO THE MEAN STRUCTURE UPON SUPERPOSITION OF THE BACKBONE ATOMS N, CA, AND C' OF RESIDUES 2-11.
FOR THE PRESENT STRUCTURES THE NMR DISTANCE CONSTRAINTS WERE WEIGHTED SUCH THAT A VIOLATION OF AN UPPER DISTANCE LIMIT OF 0.1 ANGSTROM CORRESPONDS TO AN ENERGY OF KT/2. THE CONSTRAINTS ON DIHEDRAL ANGLES RESULTING FROM MEASUREMENTS OF VICINAL COUPLING CONSTANTS WERE WEIGHTED SUCH THAT A VIOLATION OF 2.5 DEGREES CORRESPONDS TO AN ENERGY OF KT/2.
TORSION ANGLE DYNAMICS, RESTRAINED ENERGY MINIMISATION
PEPTIDE TOXIN, NEUROTOXIN, NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR ANTAGONIST, ALPHA-CONOTOXIN, NMR SOLUTION STRUCTURE