DELTA-R307A DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE AND CARBON DIOXIDE

Kinetic and Crystallographic Studies on Deacetoxycephalosporin C Synthase (Daocs)

Studies on the Active Site of Deacetoxycephalosporin C Synthase (Daocs)

MDTTVPTFSLAELQQGLHQDEFRRCLRDKGLFYLTDCGLTDTELKSAKDIVIDFFEHGSEAEKRAVTSPVPTMRRGFTGL
ESESTAQITNTGSYSDYSMCYSMGTADNLFPSGDFERIWTQYFDRQYTASRAVAREVLRATGTEPDGGVEAFLDCEPLLR
FRYFPQVPEHRSAEEQPLRMAPHYDLSMVTLIQQTPCANGFVSLQAEVGGAFTDLPYRPDAVLVFCGAIATLVTGGQVKA
PRHHVAAPRRDQIAGSSRTSSVFFLRPNADFTFSVPLARECGFDVSLDGETATFQDWIGGNYVNIRRA

100 MM HEPES-NAOH, PH 7.0, 0.5 MM PB(OAC)2(OH2)2, 5 MM NA2SUCCINATE, 5 MM NAHCO3, GLYCEROL 5-10% (W/V), 1.4-1.6 M AMMONIUM SULPHATE

DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH FE(II)

DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH FE(II) AND 2-OXOGLUTARATE

DEACETOXYCEPHALOSPORIN C SYNTHASE FROM S. CLAVULIGERUS

DELTA-R306 DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH IRON AND 2-OXOGLUTARATE.

CHAIN A ENGINEERED MUTATION THR308ALA. THE C-TERMINAL RESIDUES
SER, LYS, ALA ARE DELETED.

FUNCTION: DAOCS CATALYZES THE STEP FROM PENICILLIN N TO DEACETOXY-
CEPHALOSPORIN C.
COFACTOR: IRON AND ASCORBATE.
PATHWAY: BIOSYNTHESIS OF CEPHALOSPORIN ANTIBIOTICS.
SIMILARITY: BELONGS TO THE IRONULLSCORBATE-DEPENDENT FAMILY OF
OXIDOREDUCTASES. STRONG, TO CEFF.

DATA DETWINNED USING CCP4 PROGRAMME DETWIN (A. LESLIE)

BIOLOGICALUNIT: ACTIVE AS A MONOMER, TRIMERIC ASSEMBLYOBSERVED IN THE CRYSTAL.

FERROUS OXYGENASE, CEPHALOSPORIN, 2-OXOGLUTARATE, C-TERMINUS ANTIBIOTICS, OXIDOREDUCTASE, OXIDATIVE COUPLING CONTROL