Structural and mechanistic basis of porphyrin metallation by ferrochelatase.

SRKKMGLLVMAYGTPYKEEDIERYYTHIRRGRKPEPEMLQDLKDRYEAIGGISPLAQITEQQAHNLEQHLNEIQDEITFK
AYIGLKHIEPFIEDAVAEMHKDGITEAVSIVLAPHFSTFSVQSYNKRAKEEAEKLGGLTITSVESWYDEPKFVTYWVDRV
KETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTRD
LFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMPNAKPEFIDALATVVLKKLGR

PEG2000, MAGNESIUM CHLORIDE, TRIS, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K

THE SAME PROTEIN AT LOWER RESOLUTION, DOES NOT SHOW COMPLEXED MAGNESIUMM HYDRATES

THE SAME PROTEIN IN COMPLEX WITH N-METHYL MESOPORPHYRIN, A SUBSTRATE ANALOGUE

THE SAME PROTEIN IN COMPLEX WITH CU(II)-MESOPORPHYRIN, A PRODUCT ANALOGUE