SOLUTION STRUCTURE OF BEF3-ACTIVATED CHEY FROM ESCHERICHIA COLI
Beryllofluoride Mimics Phosphorylation of NtrC and Other Bacterial Response Regulators
MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSA LPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM
THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY.
structures with the least restraint violations
THE STRUCTURES ARE BASED ON A TOTAL OF 972 NON-TRIVIAL NOE RESTRAINTS (213 INTRA RESIDUE, 271 SEQUENTIAL, 238 MEDIUM-RANGE, AND 250 LONG-RANGE). 78 PHI TORSION ANGLE RESTRAINTS AND 17 CHI1 RESTRAINTS WERE USED. 47 HYDROGEN BONDS ( 94 UPPER AND LOWER DISTANCE RESTRAINTS) WERE USED IN LATER STAGES OF REFINEMENT.
4 MM CHEY U-15N, 13C, 16 MM BECL2, 100 MM NAF, 20 MM MGCL2, PH 6.7
3 MM U-15N, 16 MM BECL2, 100 MM NAF, 20 MM MGCL2, PH 6.7
3 MM U-10% 13C, 16 MM BECL2, 100 MM NAF, 20 MM MGCL2, PH 6.7
2 MM CHEY, 16 MM BECL2, 100 MM NAF, 20 MM MGCL2, PH 6.7
DELAGLIO, BRZESIEK, VUISTER, ZHU, PFEIFER, BAX
BARTELS, XIA, BILLETER, GUNTERT, WUTHRICH
BEFX, CHEY, RESPONSE REGULATOR, CHEMOTAXIS, TWO-COMPONENT, SIGNALING PROTEIN