SOLUTION STRUCTURE OF A URACIL CONTAINING HAIRPIN DNA
Structural basis for uracil DNA glycosylase interaction with uracil: NMR study.
Custom made by Ransom Hill Biosciences, Inc.,Ramona CA (USA)
This structure was determined using standard 2D homonuclear techniques
structures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the least restraint violations,structures with the lowest energy
The structures are based on a total of 225 restraints, 145 are NOE-derived distance constraints, 64 dihedral aangle restraints, 16 distance restraints from hydrogen bonds.
Restrained molecular dynamics and Energy minimization.
5 mM oligo; no buffer; pH = 7.0 Temp = 305K; 99% D2O
5 mM oligo; no buffer; pH = 7.0 Temp = 305K; 90% H20 +10% D2O
Uracil, Hairpin DNA, Hairpin loop, Double helix, UDG-Uracil interaction, DNA