SOLUTION STRUCTURE OF THE FORKHEAD DOMAIN OF THE ADIPOCYTE-TRANSCRIPTION FACTOR FREAC-11 (S12)
Solution structure and dynamics of the DNA-binding domain of the adipocyte-transcription factor FREAC-11.
MLVKPPYSYIALITMAIQNAPEKKITLNGIYQFIMDRFPFYRENKQGWQNSIRHNLSLNECFVKVPRDDKKPGKGSYWTL DPDSYNMFENGSFL
HNCA, HN(CO)CA, HNCACB, CACB(CO)NH AND HNCO EXPERIMENTS WERE USED TO OBTAIN SEQUENTIAL ASSIGNMENTS FOR THE BACKBONE. SIDE-CHAIN RESONANCES WERE ASSIGNED USING TOCSY-15N-HSQC, C(CO)NH, H(CCO)NH AND HC(C)H-TOCSY EXPERIMENTS.
CROSS RELAXATION BETWEEN 13CA-1HA DIPOLAR AND 13C' CHEMICAL SHIFT ANISOTROPY
CROSS RELAXATION BETWEEN 13CA-1HA AND 15N-1HN DIPOLAR INTERACTIONS
THE STRUCTURE WAS DETERMINED FROM 1042 DISTANCE RESTRAINTS, COMPLEMENTED WITH 145 RESTRAINTS ON DIHEDRAL ANGLES AND 36 RESTRAINTS ON HYDROGEN BOND LENGTHS.
COMBINED DISTANCE GEOMETRY/SIMULATING ANNEALING PROCEDURE
1 MM S12 DBD, 10 MM NACL, 5 MM MGCL2, 0.02% NAN3, 5 MM DTT
WINGED HELIX, DNA-RECOGNITION HELIX, GENE REGULATION