SOLUTION STRUCTURE OF A MINI-PROTEIN REPRODUCING THE CORE OF THE CD4 SURFACE INTERACTING WITH THE HIV-1 ENVELOPE GLYCOPROTEIN
Rational engineering of a miniprotein that reproduces the core of the CD4 site interacting with HIV-1 envelope glycoprotein.
SYNTHETIC MINIPROTEIN (SOLID PHASE METHOD USING FMOC-PROTECTED AMINO ACIDS)
ADDITIONAL EXPERIMENTS (2D NOESY, 2D DQF-COSY) RECORDED AT 35 C
THE STRUCTURE IS BASED ON A TOTAL OF 239 DISTANCE RESTRAINTS, 46 ANGULAR RESTRAINTS, AND 9 ADDITIONAL RESTRAINTS FOR THE DISULFIDE BRIDGES
closest to the average, fewest violations, lowest energy
ALPHA-BETA STRUCTURE, CHARYBDOTOXIN-LIKE MOTIF, BINDING PROTEIN