CRYSTAL STRUCTURE OF THE 30 KDA FRAGMENT OF E. COLI DNA TOPOISOMERASE I. HEXAGONAL FORM

Conformational changes in E. coli DNA topoisomerase I.

30 KDA FRAGMENT COMPRISING DOMAINS II AND III

FVPEEFWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAA
STRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDV
NVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAV
NKGDALTLVELTPAQHFTKPPARF

2M AMMONIUM SULFATE, 100MM HEPES PH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 3'-5'-ADENOSINE DIPHOSPHATE

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'PTPTPT

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH TPTPTP3'

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'PTPTPTP3'

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'-THYMIDINE MONOPHOSPHATE

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 3'-THYMIDINE MONOPHOSPHATE

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'-THYMIDINE MONOPHOSPHATE AND 3'- THYMIDINE MONOPHOSPHATE

CRYSTAL STRUCTURE OF THE 30 KDA FRAGMENT OF E. COLI DNA TOPOISOMERASE I. MONOCLINIC FORM

MOST OF THE REFINEMENT DONE WITH REFMAC. SOLVENT CORRECTION WITH XPLOR. FINAL REFINEMENT WITH CNS 0.9
R. A. ENGH AND R. HUBER, ACTA CRYST. SECT. A., 1991

DNA TOPOISOMERASE, DECATENATING ENZYME, ISOMERASE