SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR
Solution structure of an RNA duplex including a C-U base pair.
Structure model and physicochemical properties of the C-U mismatch pair in the double stranded RNA in solution
RNA (5'-R(*CP*GP*AP*CP*UP*CP*AP*GP*G)-3')
RNA (5'-R(*CP*CP*UP*GP*CP*GP*UP*CP*G)-3')
THIS OLIGONUCLEOTIDE WAS CHEMICALLY SYNTHESIZED (PHOSPHORAMIDITE METHOD).
THIS STRUCTURE WAS DETERMINED USING STANDARD 2D HOMONUCLEAR TECHNIQUES
SUMMATION OF TOTAL ENERGY (COVALENT GEOMETRY, NON-BOND ENERGY, DISTANCE AND DIHEDRAL ANGLE CONSTRAINTS)
DISTANCE CONSTRAINTS (NOE 433, HYDROGEN BOND (BASE-PAIR) 54), DIHEDRAL ANGLE CONSTRAINTS 45
R[CGACUCAGG]_R[CCUG(15N4)CGUCG] (AMINO-NITROGEN OF CYTIDINE IS POINT-LABELED (> 95%))