HEPATITIS C VIRUS NS5B RNA-DEPENDENT RNA POLYMERASE

Crystal structure of the RNA-dependent RNA polymerase from hepatitis C virus reveals a fully encircled active site.

ASHHHHHHSYTWTGALITPCAAEESKLPINALSNSLLRHHN(MSE)VYATTSRSAGLRQKKVTFDRLQVLDDHYRDVLKE
(MSE)KAKASTVKAKLLSVEEACKLTPPHSAKSKFGYGAKDVRNLSSKAVNHIHSVWKDLLEDTVTPIDTTI(MSE)AKN
EVFCVQPEKGGRKPARLIVFPDLGVRVCEK(MSE)ALYDVVSTLPQVV(MSE)GSSYGFQYSPGQRVEFLVNTWKSKKNP
(MSE)GFSYDTRCFDSTVTENDIRVEESIYQCCDLAPEARQAIKSLTERLYIGGPLTNSKGQNCGYRRCRASGVLTTSCG
NTLTCYLKASAACRAAKLQDCT(MSE)LVNGDDLVVICESAGVQEDAASLRAFTEA(MSE)TRYSAPPGDPPQPEYDLEL
ITSCSSNVSVAHDASGKRVYYLTRDPTTPLARAAWETARHTPVNSWLGNII(MSE)YAPTLWAR(MSE)IL(MSE)THFF
SILLAQEQLEKALDCQIYGACYSIEPLDLPQIIERLHGLSAFSLHSYSPGEINRVASCLRKLGVPPLRVWRHRARSVRAR
LLSQGGRAATCGKYLFNWAVKTKLKLTPIPAASQLDLSGWFVAGYSGGDIYHSLSRARPR

ASHHHHHHSYTWTGALITPCAAEESKLPINALSNSLLRHHNMVYATTSRSAGLRQKKVTFDRLQVLDDHYRDVLKEMKAK
ASTVKAKLLSVEEACKLTPPHSAKSKFGYGAKDVRNLSSKAVNHIHSVWKDLLEDTVTPIDTTIMAKNEVFCVQPEKGGR
KPARLIVFPDLGVRVCEKMALYDVVSTLPQVVMGSSYGFQYSPGQRVEFLVNTWKSKKNPMGFSYDTRCFDSTVTENDIR
VEESIYQCCDLAPEARQAIKSLTERLYIGGPLTNSKGQNCGYRRCRASGVLTTSCGNTLTCYLKASAACRAAKLQDCTML
VNGDDLVVICESAGVQEDAASLRAFTEAMTRYSAPPGDPPQPEYDLELITSCSSNVSVAHDASGKRVYYLTRDPTTPLAR
AAWETARHTPVNSWLGNIIMYAPTLWARMILMTHFFSILLAQEQLEKALDCQIYGACYSIEPLDLPQIIERLHGLSAFSL
HSYSPGEINRVASCLRKLGVPPLRVWRHRARSVRARLLSQGGRAATCGKYLFNWAVKTKLKLTPIPAASQLDLSGWFVAG
YSGGDIYHSLSRARPR

PEG 4000, GLYCEROL, DTT, MES, NACL, pH 5, BATCH, temperature 22K

RNA-DEPENDENT RNA POLYMERASE (E.C.2.7.7.48)

POLYMERASE RNA-DEPENDENT RNA POLYMERASE HEPATITIS C VIRUS NS5B APOENZYME, TRANSFERASE

SYTWTGALITPCAAEESKLPINALSNSLLRHHNMVYATTSRSAGLRQKKVTFDRLQVLDDHYRDVLKEMKAKASTVKAKL
LSVEEACKLTPPHSAKSKFGYGAKDVRNLSSKAVNHIHSVWKDLLEDTVTPIDTTIMAKNEVFCVQPEKGGRKPARLIVF
PDLGVRVCEKMALYDVVSTLPQVVMGSSYGFQYSPGQRVEFLVNTWKSKKNPMGFSYDTRCFDSTVTENDIRVEESIYQC
CDLAPEARQAIKSLTERLYIGGPLTNSKGQNCGYRRCRASGVLTTSCGNTLTCYLKASAACRAAKLQDCTMLVNGDDLVV
ICESAGTQEDAASLRVFTEAMTRYSAPPGDPPQPEYDLELITSCSSNVSVAHDASGKRVYYLTRDPTTPLARAAWETARH
TPVNSWLGNIIMYAPTLWARMILMTHFFSILLAQEQLEKALDCQIYGACYSIEPLDLPQIIERLHGLSAFSLHSYSPGEI
NRVASCLRKLGVPPLRVWRHRARSVRARLLSQGGRAATCGKYLFNWAVKTKLKLTPIPAASRLDLSGWFVAGYSGGDIYH
SLSRARPR