Structure and Mutagenesis of the Dbl Homology Domain

MKGFDTTAINKSYYNVVLQNILETENEYSKELQTVLSTYLRPLQTSEKLSSANISYLMGNLEEICSFQQMLVQSLEECTK
LPEAQQRVGGCFLNLMPQMKTLYLTYCANHPSAVNVLTEHSEELGEFMETKGASSPGILVLTTGLSKPFMRLDKYPTLLK
ELERHMEDYHTDRQDIQKSMAAFKNLSAQCQEVRKRKELELQILTEAIR

THE STRUCTURE WAS DETERMINED USING A COMBINATION OF TRIPLE- AND QUADRUPLE- RESONANCE NMR EXPERIMENTS ON 13C/ 15N AND PARTIALLY OR FULLY DEUTERATED SAMPLES WITH SELECTIVE METHYL LABELING AT VALINE,LEUCINE AND ISOLEUCINES.

NOESY TOCSY 3D THROUGH BOND TRANSFER EXPERIMENTS

X-PLOR 3.851 WAS USED IN COMBINATION WITH ARIA (REF. M. NILGES)
SIMULATED ANNEALING, TORSION ANGLE DYNAMICS

RHO-GTPASE EXCHANGE FACTOR, TRANSPORT PROTEIN