SUGAR RING DISTORTION IN THE GLYCOSYL-ENZYME INTERMEDIATE OF A FAMILY G/11 XYLANASE
2-DEOXY-2-FLUORO-XYLOBIOSE COVALENTLY BONDED TO GLU 78 OE2
ASTDYWQNWTDGGGIVNAVNGSGGNYSVNWSNTGNFVVGKGWTTGSPFRTINYNAGVWAPNGNGYLTLYGWTRSPLIEYY VVDSWGTYRPTGTYKGTVKSDGGTYDIYTTTRYNAPSIDGDRTTFTQYWSVRQSKRPTGSNATITFTNHVNAWKSHGMNL GSNWAYQVMATEGYQSSGSSNVTVW
ENDO-1,4-BETA-XYLANASE, 1,2-DEOXY-2-FLUORO-XYLOPYRANOSE
XYLANASE, GLYCOSIDASE, GLYCOSYL-ENZYME INTERMEDIATE, BOAT CONFORMATION, HYDROLASE, XYLAN DEGRADATION
MFKFKKNFLVGLSAALMSISLFSATASAASTDYWQNWTDGGGIVNAVNGSGGNYSVNWSNTGNFVVGKGWTTGSPFRTIN YNAGVWAPNGNGYLTLYGWTRSPLIEYYVVDSWGTYRPTGTYKGTVKSDGGTYDIYTTTRYNAPSIDGDRTTFTQYWSVR QSKRPTGSNATITFTNHVNAWKSHGMNLGSNWAYQVMATEGYQSSGSSNVTVW
THE NUCLEOPHILE TO WHICH THE INHIBITOR IS BOUND IS GLU 78.
THE ACID BASE CATALYST IS RESIDUE GLU 172.