DNA-BINDING DOMAIN OF HUMAN TELOMERIC PROTEIN, HTRF1, NMR, 18 STRUCTURES
Solution structure of the DNA-binding domain of human telomeric protein, hTRF1.
THE STRUCTURES OF HTRF1 DNA-BINDING DOMAIN WERE DETERMINED BY 2D PROTON NMR SPECTROSCOPY.
DETAILS OF THE STRUCTURE CALCULATION AND ALL STRUCTURAL STATISTICS WILL BE GIVEN IN THE REFERENCES CITED ABOVE. THE STRUCTURES WERE CONSTRUCTED ON THE BASIS OF 546 INTERPROTON DISTANCE RESTRAINTS DERIVED FROM NOES AND 27 DIHEDRAL ANGLE RESTRAINTS. THE METHOD USED TO DETERMINE THE STRUCTURES IS THE SIMULATED-ANNEALING PROTOCOL IN FOUR-DIMENSIONAL SPACE IMPLEMENTED IN THE EMBOSS PROGRAM [T.NAKAI, A.KIDERA AND H.NAKAMURA, J.BIOMOL.NMR,3,19-40(1993)]. THE STRUCTURES WERE REFINED BY THE RESTRAINED ENERGY MINIMIZATION USING AMBER ALL-ATOM FORCE FIELD.
DNA-BINDING DOMAIN, MYB REPEATS, TELOMERES, TRF
MAEDVSSAAPSPRRCADGRDADPTEEQMAETERNDEEQFECQELLECQVQVGAPEEEEEEEEDAGLVAEAEAVAAGWMLD FLCLSLCRAFRDGRSEDFRRTRNSAEAIIHGLSSLTACQLRTIYICQFLTRIAAGKTLDAQFENDERITPLESALMIWGS IEKEHDKLHEEIQNLIKIQAIAVCMENGNFKEAEEVFERIFGDPNSHMPFKSKLLMIISQKDTFHSFFQHFSYNHMMEKI KSYVNYVLSEKSSTFLMKAAAKVVESKRTRTITSQDKPSGNDVEMETEANLDTRKSVSDKQSAVTESSEGTVSLLRSHKN LFLSKLQHGTQQQDLNKKERRVGTPQSTKKKKESRRATESRIPVSKSQPVTPEKHRARKRQAWLWEEDKNLRSGVRKYGE GNWSKILLHYKFNNRTSVMLKDRWRTMKKLKLISSDSED