HEN EGG WHITE LYSOZYME WITH A ISOASPARTATE RESIDUE
N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE
Succinimide and isoaspartate residues in the crystal structures of hen egg-white lysozyme complexed with tri-N-acetylchitotriose.
Crystallography of Succinimide Hen Egg-White Lysozyme at Low Temperatures
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPC SALLSSDITASVNCAKKIVS(IAS)GNGMNAWVAWRNRCKGTDVQAWIRGCRL
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPC SALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL
CRYSTALS WERE PREPARED AT 277 K FROM THE DROPLET MIXTURE OF A 20 MG/ML PROTEIN SOLUTION CONTAINING 7 MM TRI-N-ACETYLCHITOTRIOSE (NAG3) AND 50 MM ACETATE BUFFER PH 4.2 WITH EQUAL VOLUME OF A RESERVOIR SOLUTION CONTAINING 2.0 M AMMONIUM FORMATE AND 100 MM N-(2-HYDROXYETHYL)PIPERAZINE-N'-(2-ETHANESULFONIC ACID) (HEPES) BUFFER PH 7.5. PRIOR TO THE DIFFRACTION DATA COLLECTION, THE CRYSTAL WAS SOAKED IN A CRYOPROTECTANT SOLUTION CONTAINING 30 % (W/V) POLYETHYLENE GLYCOL 20,000, 2.2 M AMMONIUM FORMATE, 100 MM HEPES BUFFER PH 7.5, AND 7 MM NAG3 FOR 2 MINUTES AT 277 K.
A BULK SOLVENT ANALYSIS, WITH MASK PARAMETERS K = 0.350 (ELECTRON/ANGSTROM**3) AND B = 73.6 (ANGSTROM**2), WAS APPLIED IN THE REFINEMENT. ALTHOUGH BOND DISTANCES BETWEEN MET 12 SD AND CE AND BETWEEN ARG 14 CG AND CD DEVIATE BY LARGER THAN 4.0*RMSD FROM THE IDEAL VALUES, ALL THE ATOMS OF MET 12 AND ARG 14 ARE DEFINED IN THE ELECTRON DENSITIES.
ISOASPARTATE, HYDROLASE, O-GLYCOSYL HYDROLASE
MRSLLILVLCFLPLAALGKVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRW WCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL