X-ray crystallography reveals stringent conservation of protein fold after removal of the only disulfide bridge from a stabilized immunoglobulin variable domain.
TPDIQMTQSPSSLSASVGDRVTITVQASQDIIKHLNWYQQTPGKAPKLLIYEASNLQAGVPSRFSGSGSGTDYTFTISSL QPEDIATYYCQQYQSLPYTFGQGTKLQIT
VAPOUR DIFFUSION AL 4 C FROM A PROTEIN SOLUTION CONTAINING 8 MG/ML IN 50 MM POTASSIUM PHOSPHATE BUFFER AL PH 7, USING 2 M AMMONIUM SULFATE IN 100 MM SODIUM ACETATE BUFFER, PH 4.6, vapor diffusion, temperature 277K
SIMULATED ANNEALING WITH X-PLOR, FINAL REFINEMENT WITH SHELXL-97
DISULFIDE-FREE, IMMUNOGLOBULIN FRAGMENT, BENCE-JONES, IMMUNOGLOBULIN
DIQMTQSPSSLSASVGDRVTITCQASQDIIKYLNWYQQTPGKAPKLLIYEASNLQAGVPSRFSGSGSGTDYTFTISSLQP EDIATYYCQQYQSLPYTFGQGTKLQITR