PENICILLIN ACYLASE COMPLEXED WITH 2,5-DIHYDROXYPHENYLACETIC ACID

Ligand-induced conformational change in penicillin acylase.

Penicillin acylase has a single-amino-acid catalytic centre.

Expression, purification and crystallization of penicillin G acylase from Escherichia coli ATCC 11105.

Structural Studies of Penicillin Acylase

EQSSSEIKIVRDEYGMPHIYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPDAIRA
QIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDVAMIFVGTMANRFSDSTSEIDNLALLT
ALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAVQESNYPLKFNQQNSQTA

SNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAE
RLSAEKPGYYLHNGKWVKMLSREETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKA
KNWQQWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNPQSGYIANW
NNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLV
ETLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAV
QGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR

CRYSTALLIZED FROM 10% PEG 8000, 50MM MOPS, PH 7.2, STREAK SEEDED. SOAKED IN 10MM 2,5-DIHYDROXYPHENYLACETIC ACID, streak seeded

THERE IS DISORDER IN THE LIGAND WHICH IS ONLY 50% OCCUPIED.

ISOMORPHOUS TO NATIVE

THERE IS DISORDER IN A 145 AND A 146, BOTH HAVING DUAL CONFORMATIONS AS A RESULT OF LIGAND BINDING.

PENICILLIN AMIDOHYDROLASE, 2-(3,6-DIHYDROXYPHENYL)ACETIC ACID

ANTIBIOTIC RESISTANCE, LIGAND INDUCED CONFORMATIONAL CHANGE, HYDROLASE