THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY

3-D structure of the D153G mutant of Escherichia coli alkaline phosphatase: an enzyme with weaker magnesium binding and increased catalytic activity.

Crystallographic Analysis of Reversible Metal Binding Observed in a Mutant (Asp 153--> Gly) of E. Coli Alkaline Phosphatase

3-D Structure of the (Asp 101-->Ser) of E.Coli Alkaline Phosphatase with Higher Catalytic Activity

MUTANT WITH 4-FOLD INCREASED ACTIVITY AND WEAKER MG BINDING

TPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQGATPAALV
AHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREQAEARGYQLVSDA
ASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQ
VEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVM
SYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK

LAC PROMOTER. FOR MORE INFORMATION ABOUT THE EXPRESSION SYSTEM CONSULT MANDECKI ET AL. GENE 94, 103-107; (1990).
PWM528 AS BAMH1/HINDIII RESTRICTION FRAGMENT

SHEET
SHEET
DETERMINATION METHOD: KABSCH & SANDER;
SHEET_ID: S1A; SAME AS 1ALK.
SHEET_ID: S2A; SAME AS 1ALK.
SHEET_ID: S1B; SAME AS 1ALK.
SHEET_ID: S2B; SAME AS 1ALK.

FOR MORE INFORMATION ABOUT THE EXPRESSION SYSTEM CONSULT MANDECKI ET AL. GENE 94, 103-107; (1990).

MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEI
TAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAG
EWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPT
LAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVA
PDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK

TWO ZN2+ AND ONE MG2+ IONS ARE AT THE ACTIVE-SITE.