STRUCTURE OF THE REDUCED FORM OF MERP, THE PERIPLASMIC PROTEIN FROM THE BACTERIAL MERCURY DETOXIFICATION SYSTEM, NMR, 20 STRUCTURES
Structures of the reduced and mercury-bound forms of MerP, the periplasmic protein from the bacterial mercury detoxification system.
THE PROTEIN STUDIED EXCLUDES THE PRECURSOR SIGNAL SEQUENCE. THIS IS THE NATIVE FORM OF THE PROTEIN AFTER CELL PROCESSING.
REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. IN SUMMARY, INITIAL CALCULATIONS WERE STARTED FROM A LINEAR POLYPEPTIDE TEMPLATE WITH RANDOM BACKBONE ANGLES. AN ITERATIVE REFINEMENT WAS EMPLOYED USING ADDITIONAL RESTRAINTS AS THE QUALITY OF THE STRUCTURES IMPROVED AND AMBIGUITIES IN THE DATA WERE RESOLVED.
MERCURY DETOXIFICATION, PERIPLASMIC, HEAVY METAL TRANSPORT, ALPHA-BETA SANDWICH
MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKALSKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKAT ADAGYPSSVKQ
MERCURY BINDING CYS RESIDUES ARE CONTAINED IN THE GMTCAAC HEAVY METAL BINDING REGION (HMA).