PHOTOLYSED CARBONMONOXY-MYOGLOBIN AT 20 K
Crystal structure of photolysed carbonmonoxy-myoglobin.
Crystal Structure of Myoglobin from a Synthetic Gene
High-Level Expression of Sperm Whale Myoglobin in Escherichia Coli
HEME BOUND TO HIS-93, PHOTOLYSED CO ATOP HEME
MVLSEGEWQLVLHVWAKVEADVAGHGQDILIRLFKSHPETLEKFDRFKHLKTEAEMKASEDLKKHGVTVLTALGAILKKK GHHEAELKPLAQSHATKHKIPIKYLEFISEAIIHVLHSRHPGNFGADAQGAMNKALELFRKDIAAKYKELGYQG
DATA WERE COLLECTED UNDER CONTINUOUS ILLUMINATION USING AN OPEN FLOW HELIUM CRYOSTAT FOR COOLING.
PROTEIN WAS CRYSTALLIZED FROM 3.6 M AMMONIUM SULFATE. CRYSTALS WER SOAKED FOR 1 HOUR IN A THOROUGHLY DEGASSED AND N2 - SATURATED CRYOPROTECTANT SOLUTION MADE BY ADDITION OF 100 MG SUCROSE, 100 MG GLUCOSE AND 8 MG NA-DITHIONATE TO 1 ML OF 70% SATURATED AMMONIUM SULFATE WITH 50 MM TRIS. HCL PH 9.0. THEN THE SOLUTION WAS EXCHANGED AGAINST A CO SATURATED ONE. DISTINCT COLOR CHANGES ACCOMPANIED THE FORMATION OF UNLIGATED MYOGLOBIN FROM MET-MB AND MBCO FROM UNLIGATED MB.
HEME PARAMETERS WERE RELAXED TO ALLOW FOR REFINEMENT OF UNLIGATED, PHOTOLYSED, AND CO-BOUND COMPLEX.
OXYGEN STORAGE, INTERMEDIATE IN LIGAND BINDING, RESPIRATORY PROTEIN