STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 2-FLUOROETHANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE
Comparison of straight chain and cyclic unnatural amino acids embedded in the core of staphylococcal nuclease.
Mobile Unnatural Amino Acid Side Chains in the Core of Staphylococcal Nuclease
Interactions in Nonnative and Truncated Forms of Staphylococcal Nuclease as Indicated by Mutational Free Energy Changes
VARIANT FORMED BY CHEMICAL MODIFICATION OF THE SOLE CYSTEINE RESIDUE
ATSTKKLHKEPATLIKAIDGDT(EFC)KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEF DKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ
ATSTKKLHKEPATLIKAIDGDTCKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQ RTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ
10 MM POTASSIUM PHOSPHATE, PH 8.15 PROTEIN CONCENTRATION = 2 MGS/ML MPD =21% T=4 DEGREES C, temperature 277K
NUCLEASE, UNNATURAL AMINO ACID, HYDROLASE, ENDONUCLEASE
MLVMTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCLVLTLVVVSSLSSSANASQTDNGVNRSGSEDPTV YSATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDK GQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ