Discrepancies between the NMR and X-ray structures of uncomplexed barstar: analysis suggests that packing densities of protein structures determined by NMR are unreliable.
MKKAVINGEQIRSISDLHQTLKKELALPEYYGENLDALWDCLTGWVEYPLVLEWRQFEQSKQLTENGAESVLQVFREAKA EGADITIILS
1BGS & 2BRS ARE THE COMPLEX OF BARSTAR WITH BARNASE
C82A MUTANT WAS CRYSTALLIZED FROM 40-60% AMMONIUM SULFATE, 50MM PHOSPHATE, PH=6.5. PROTEIN CONC=45 MG/ML. C40A AND DTNB LABELLED CRYSTALS ALSO CRYSTALLIZED BUT DID NOT DIFFRACT TO HIGH RESOLUTION.
MET PRESENT AT N-TERMINUS DURING EXPRESSION IS NOT INCLUDED IN THE SEQUENCE OR THE STRUCTURE.
RESTRAINED, 100 KCAL/MOL WEIGHT FINAL POSITIONAL REFINEMENT USING ALL REFLECTIONS AND REMOVING NCS RESTRAINTS.
BARSTAR, C82A, DIMER, UNCOMPLEXED, RIBONUCLEASE INHIBITOR
KKAVINGEQIRSISDLHQTLKKELALPEYYGENLDALWDCLTGWVEYPLVLEWRQFEQSKQLTENGAESVLQVFREAKAE GCDITIILS