BRANCHPOINT HELIX FROM YEAST AND BINDING SITE FOR PHAGE GA/MS2 COAT PROTEINS, NMR, 12 STRUCTURES
NMR structure and dynamics of an RNA motif common to the spliceosome branch-point helix and the RNA-binding site for phage GA coat protein.
RBS AND START SITE FOR PHAGE GA REPLICASE GENE
IN VITRO SYNTHESIS FROM DNA TEMPLATE USING T7 RNA POLYMERASE. HAIRPIN CORRESPONDS TO NT -16 - +5 OF PHAGE GA REPLICASE AND THE YEAST PRE-MRNA BRANCHPOINT HELIX
FURTHER DETAILS OF DATA ACQUISITION AND STRUCTURE CALCULATION CAN BE FOUND IN THE METHODS SECTION OF THE MANUSCRIPT LISTED ABOVE. MODELS 1-6 ARE MINIMIZED STRUCTURES CALCULATED WITHOUT BASE PAIR CONSTRAINTS FOR A6, A7, AND U16. MODELS 7-12 ARE MINIMIZED STRUCTURES CALCULATED USING A6-U16 BASE PAIR CONSTRAINTS AND AN A7 N1H-U16 O2 HYDROGEN BOND.
REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE.
BRANCHPOINT HELIX, PHAGE MS2, BULGE, BASE TRIPLE, RIBONUCLEIC ACID, RNA
A-A)*U MOTIF. PKA OF A7~6.1 AND IS PARTIALLY PROTONATED AT PH 6.8, A7 NH1 MAY BE STABILIZED BY U16 O2 (MODELS 7-12).